Immunostaining of an “8”-shaped-hatching blastocyst at 120 h post-insemination.

<p>The images show ZO2 (red) and Na⁺/K⁺-ATPase (green) staining, nuclei stained with Hoechst (blue), and the midplane section (<b>A–D</b>) and maximum intensity projection (<b>E–H</b>). The two TE-structure proteins Na⁺/K⁺-ATPase and ZO2 were similarly expressed inside and outside the ZP. Scale bar, 20 μm.